KMID : 0360220120530020323
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Journal of the Korean Ophthalmological Society 2012 Volume.53 No. 2 p.323 ~ p.332
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The Antifibrotic Effects of ¥á-Tocotrienols in Primary Cultured Orbital Fibroblasts from Thyroid-Associated Ophthalmopathy Patients
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Kim Suk-Jin
Kang Haeng-Ku Kang Sung-Mo
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Abstract
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Purpose: To evaluate the antiproliferative and antifibrotic effects of ¥á-tocotrienols in primary cultured orbital fibroblasts from thyroid-associated ophthalmopathy (TAO) patients.
Methods: Orbital fibroblasts were cultured (5 eyes from TAO patients, 5 eyes from normal patients) and classified into a control group, ¥á-tocotrienol group and ¥á-tocopherol group. The cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay. The proliferation of orbital fibroblasts was measured using the Click-iTTM assay. The collagen production of the control and ¥á-tocotrienol groups was measured using a hydroxyproline assay.
Results: The ¥á-tocotrienol and ¥á-tocopherol groups showed no cytotoxicity up to 150 ¥ìm in orbital fibroblasts from TAO and normal patients. The proliferation of orbital fibroblasts from TAO and normal patients was significantly inhibited with ¥á -tocotrienol at 80 ¥ìm and 120 ¥ìm. The collagen production of orbital fibroblasts from TAO patients was significantly inhibited with ¥á-tocotrienol at 120 ¥ìm.
Conclusions: The results from the present study indicate that non-toxic concentrations of ¥á-tocotrienol have significant antiproliferative and antifibrotic effects on orbital fibroblasts from TAO patients.
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KEYWORD
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¥á-tocopherol, ¥á-tocotrienol, Fibrosis, Orbital fibroblast, Thyroid-associated ophthalmopathy
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